Amylase from Lactobacillus cellobiosus D‐39 isolated from vegetable wastes: Purification and characterization

An organism producing α‐amylase and identified as Lactobacillus cellobiosus D‐39 was recently isolated from vegetable wastes. Its amylase was purified by (NH 4 ) 2 SO 4 precipitation and DEAE cellulose column chromatography and was obtained in crystalline form. It was fairly stable at a broadly neutral pH range and maximally active at 50°C. The molecular weight was 22 500 daltons as determined by SDS gel electrophoresis.