A method is described by which immune systems can be studied at subcellular level with the electron microscope, using a ferritin-antibody conjugate. A conjugated immune serum to Staphylococcus aureus was applied to homologous organisms, embedded in methacrylate with uranium and thin sections cut. An antigen-antibody reaction was detected by attachment of the ferritin molecule in the capsule-like material. Use of formalin in primary fixation for electron microscopy as well as nonspecific immunofluorescence is discussed.
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Smith et al. (1960) studied this question.