Recursive PCR: a novel technique for total gene synthesis

Chrisostomos Prodromou; Laurence H. Pearl

doi:10.1093/protein/5.8.827

January 1, 1992Protein Engineering Design and SelectionOpen Access

Recursive PCR: a novel technique for total gene synthesis

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Key Points

To develop and evaluate recursive polymerase chain reaction (PCR) as an efficient method for total de novo gene synthesis.
Assembles overlapping synthetic oligonucleotides into full-length gene constructs using iterative cycles of polymerase chain reaction amplification.
Demonstrates successful de novo assembly and amplification of complete gene sequences directly from short oligonucleotide building blocks.
Provides a streamlined alternative to traditional multi-step restriction enzyme digestion and ligation techniques.

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Implication

Methodological report demonstrates total gene synthesis using recursive polymerase chain reaction, highlighting an efficient strategy for constructing full-length synthetic genes.

Abstract

Chrisostomos Prodromou, Laurence H. Pearl; Recursive PCR: a novel technique for total gene synthesis, Protein Engineering, Volume 5, Issue 8, 1 December 19

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Prodromou et al. (1992) studied this question.

synapsesocial.com/papers/6a7c8621c3ed8adf7a6dcf33 https://doi.org/10.1093/protein/5.8.827

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Recursive PCR: a novel technique for total gene synthesis

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Recursive PCR: a novel technique for total gene synthesis

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