In the assay of multicomponent systems by ultraviolet spectrophotometry, it is possible to improve accuracy and precision and also to obtain various statistical criteria by considering data over the range of wavelengths concerned. A linear least squares approach has been programmed to analyse mixtures of drugs in the presence or absence of known background. When the background was unknown but could be assumed of simple form, a non-linear alternative was successful.
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Madsen et al. (1974) studied this question.
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