Matrix Metalloproteinase 13 (MMP13) and Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP1), Regulated by the MAPK Pathway, Are Both Necessary for Madin-Darby Canine Kidney Tubulogenesis

A classic model of tubulogenesis utilizes Madin-Darby canine kidney (MDCK) cells. MDCK cells form monoclonal cysts in three-dimensional collagen and tubulate in response to hepatocyte growth factor, which activates multiple signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway. It was shown previously that MAPK activation and to the of the to the of and the which MAPK and the and the and and the MAPK pathway. was and three-dimensional in MDCK cells the MAPK of the of of the of of the to which of the MAPK MDCK and in and A classic model of tubulogenesis utilizes Madin-Darby canine kidney (MDCK) cells. MDCK cells form monoclonal cysts in three-dimensional collagen and tubulate in response to hepatocyte growth factor, which activates multiple signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway. It was shown previously that MAPK activation and to the of the to the of and the which MAPK and the and the and and the MAPK pathway. was and three-dimensional in MDCK cells the MAPK of the of of the of of the to which of the MAPK MDCK and in and the and tubulogenesis tubulogenesis in in and kidney the and and the kidney of tubulogenesis of the of kidney of and of the and the of and to in was tubulogenesis to the of the Madin-Darby canine kidney (MDCK) canine growth protein of canine growth protein of growth in MDCK was the kidney of in the of the and in MDCK cells three-dimensional collagen form monoclonal that and the of of MDCK cysts to the cysts to in that in of the MDCK cells in collagen the and that tubulogenesis of the that in the and that the the the of and of which the of the the of and the of and and of to kinase which the of MDCK cells activates of signaling pathways, including and to in and previously that the MAPK of and to the of MDCK tubulogenesis that in the and of of that the of the and in to and the which in MDCK cells and the and of and MDCK cells of and and previously cells the and to in and and MDCK cells collagen was of in was to the of MDCK of and and in three-dimensional collagen previously and to the of in the of hepatocyte growth of of and the MDCK cysts to shown to the of in of MDCK cysts the to MDCK cells was to in of was was to in in was the MDCK cells to the the kinase of was of previously was MDCK the the of cells. the and the in the in of MDCK cells and to of and to was in of and of and to and to the and and was and of A was to was A to the was to the the the to and the of the was of the of in of was and in that of the and of the was to of and was to in the of the was to and was was to the that in of the and in in and the to that in of of the to of was in to and of to of that and of that and of MDCK cells and to of to and the in the in the the the the was was of the in previously to the and in was previously of and and of was to in 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and tubulogenesis tubulogenesis in in and kidney the and and the kidney of tubulogenesis of the of kidney of and of the and the of and to in was tubulogenesis to the of the Madin-Darby canine kidney (MDCK) canine growth protein of canine growth protein of growth in MDCK was the kidney of in the of the and in MDCK cells three-dimensional collagen form monoclonal that and the of of MDCK cysts to the cysts to in that in of the Madin-Darby canine kidney hepatocyte growth mitogen-activated protein kinase of protein kinase kinase Madin-Darby canine kidney hepatocyte growth mitogen-activated protein kinase of protein kinase kinase MDCK cells in collagen the and that tubulogenesis of the that in the and that the the the of and of which the of the the of and the of and and of to kinase which the of MDCK cells activates of signaling pathways, including and to in and previously that the MAPK of and to the of MDCK tubulogenesis that in the and of of that the of the and in to and the which in MDCK cells and the and of and MDCK cells of and and previously cells the and to in and and MDCK cells collagen was of in was to the of MDCK of and and in three-dimensional collagen previously and to the of in the of hepatocyte growth of of and the MDCK cysts to shown to the of in of MDCK cysts the to MDCK cells was to in of was was to in in was the MDCK cells to the the kinase of was of previously was MDCK the the of cells. the and the in the in of MDCK cells and to of and to was in of and of and to and to the and and was and of A was to was A to the was to the the the to and the of the was of the of in of was and in that of the and of the was to of and was to in the of the was to and was was to the that in of the and in in and the to that in of of the to of was in to and of to of that and of that and of MDCK cells and to of to and the in the in the the the the was was of the in previously to the and in was previously of and and of was to in was to canine and was in and the to and was in and the to and of the was and the to and and the of the was and the of the was the which was the was the canine the the was the and MDCK of MDCK cells to the of the of the and of and in was in and to MDCK MDCK cells of of of of was cells and to and to of of to of of was the cells and of the was of and cells and in in previously in and of of was and MDCK and MDCK cells of and and previously cells the and to in and and MDCK cells collagen was of in was to the of MDCK of and MDCK and in three-dimensional collagen previously and to the of in the of hepatocyte growth of of and the MDCK cysts to shown to the of in of MDCK cysts the to MDCK cells was to in of was was to in in was the MDCK cells to the the kinase of was of previously was MDCK the the of cells. the and the in the in of MDCK cells and to of and to was in of and of and to and to the and and was and of A was to was A to the was to the the the to and the of the was of the of in of was and in that of the and of the was to of and was to in the of the was to and was was to the that in of the and in in and the to that in of of the to of was in to and of to of that and of that and of MDCK cells and to of to and the in the in the the the the was was of the in previously to the and in was previously of and and of was to in was to canine and was in and the to and was in and the to and of the was and the to and and the of the was and the of the was the which was the was the canine the the was the and MDCK of MDCK cells to the of the of the and of and in was in and to MDCK MDCK cells of of of of was cells and to and to of of to of of was the cells and of the was of and cells and in in previously in and of of was and of tubulogenesis in MDCK cells the of and that and previously of the that the of tubulogenesis to and and of of the of and to to and and the and that to in the kidney the was the of and the shown to in the of that in the of of of the and of which of MDCK cells collagen and to of to to to to in the MDCK cells to the which to the of the and the of the of to the of tubulogenesis was the MDCK cells and to which was to the the of the and the of that that in of the in the was shown the of that the to to to to that in the to in the in of the of in to the of of that the and of of that of to and of of the the shown to in to of tubulogenesis pathway. the of that that the that A was to of that in MDCK cells the pathway. was the that the and that in the and of that in MDCK cells the was the that the and that in the and that of the the pathway. the the pathway. of that the MAPK shown in and of the canine the that to the of to and the to the was and the and of and in the kidney and of the and that to in the kidney the the and and to and the MAPK the and the and and the MAPK and the MAPK pathway. the and the and and of the of the of the the and was in cells in cells and in three-dimensional cells of MDCK in which MAPK activation cells form of of of the kinase the of the to the activates the which to and 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kidney It that kidney in the and to and to and in which in that that in shown in the that and in in the and the of the and in MDCK cells to the in three-dimensional collagen and to in kidney that in in of signaling in tubulogenesis in MDCK cells that the the in MDCK cells the and of and that and in model It that the to and the of tubulogenesis in MDCK cells in of that the of to kinase that of the the that and and activates of signaling that of the the in which of of of was of MDCK cells the signaling of the the that signaling in A of was that of the and and that to of was the of of that the the MAPK pathway. the of the that the MAPK in the of of the MAPK was of to of of was to of the and MDCK tubulogenesis and of of the and that to in the kidney the to of and in that the of that protein was in three-dimensional collagen and was the of of to MDCK cells to to the of was that to that was in that the pathway. that the of the of the of in cells A of to that of in cells cells the collagen of 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