Laboratory study demonstrates enhanced spectrophotometric determination of chymotrypsin, trypsin, and thrombin, indicating reliable trace detection in complex enzyme mixtures.
Key Points
To modify and extend an established spectrophotometric assay for the sensitive and specific quantification of chymotrypsin, trypsin, and thrombin.
Modified the Schwert and Takenaka spectrophotometric assay using synthetic substrates N-benzoyl-L-tyrosine ethyl ester and α-p-toluenesulphonyl-L-arginine methyl ester.
Extended the spectrophotometric methodology to develop an analogous quantitative assay for thrombin.
Substantially improved assay sensitivity and specificity for differentiating closely related proteolytic enzymes.
Enabled the reliable measurement of trace amounts of chymotrypsin in the presence of excess trypsin and vice versa.