A method is described for the enumeration of bifidobacteria in faeces which consists of a dilution count on a semi-selective medium followed by assay of the amount of acetic acid produced under standard conditions. With one exception ( Lactobacillus reuteri ) the lactobacilli always produced less acetic acid than did the bifidobacteria. L. reuteri could be distinguished from the bifidobacteria by the high concentration of acetaldehyde which it produced.
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Cole et al. (1989) studied this question.
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