Key result
Only elevated circulating miR-29a correlates with both hypertrophy and fibrosis in HCM.
Why the study?
To determine whether circulating microRNAs involved in myocardial remodeling are differentially expressed in hypertrophic cardiomyopathy and correlate with left ventricular hypertrophy and fibrosis.
Are circulating miRNAs differentially expressed in HCM patients and do they correlate with hypertrophy and fibrosis?
Case-Control (n=82)
Are circulating miRNAs differentially expressed in HCM patients and do they correlate with hypertrophy and fibrosis?
Circulating miR-29a is significantly increased in HCM patients and correlates with both left ventricular hypertrophy and fibrosis, suggesting its potential as a biomarker for myocardial remodeling.
May indicate remodeling severity in HCM; leaves open biomarker utility pending validation.
OBJECTIVES: The purpose of this paper was to determine whether microRNAs (miRNAs) involved in myocardial remodeling were differentially expressed in the blood of hypertrophic cardiomyopathy (HCM) patients, and whether circulating miRNAs correlated with the degree of left ventricular hypertrophy and fibrosis. BACKGROUND: miRNAs-small, noncoding ribonucleic acids (RNAs) that regulate gene expression by inhibiting RNA translation-modulate cellular function. Myocardial miRNAs modulate processes such as cardiomyocyte (CM) hypertrophy, excitation-contraction coupling, and apoptosis; non-CM-specific miRNAs regulate myocardial vascularization and fibrosis. Recently, the possibility that circulating miRNAs may be biomarkers of cardiovascular disease has been raised. METHODS: Forty-one HCM patients were characterized with conventional transthoracic echocardiography and cardiac magnetic resonance. Peripheral plasma levels of 21 miRNAs were assessed by quantitative real-time polymerase chain reaction and were compared with levels in a control group of 41 age- and sex-matched blood donors. RESULTS: Twelve miRNAs (miR-27a, -199a-5p, -26a, -145, -133a, -143, -199a-3p, -126-3p, -29a, -155, -30a, and -21) were significantly increased in HCM plasma. However, only 3 miRNAs (miR-199a-5p, -27a, and -29a) correlated with hypertrophy; more importantly, only miR-29a correlated also with fibrosis. CONCLUSIONS: Our data suggest that cardiac remodeling associated with HCM determines a significant release of miRNAs into the bloodstream: the circulating levels of both cardiac- and non-cardiac-specific miRNAs are significantly increased in the plasma of HCM patients. However, correlation with left ventricular hypertrophy parameters holds true for only a few miRNAs (i.e., miR-199a-5p, -27a, and -29a), whereas only miR-29a is significantly associated with both hypertrophy and fibrosis, identifying it as a potential biomarker for myocardial remodeling assessment in HCM.
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Roncarati et al. (2013) conducted a case-control in Hypertrophic cardiomyopathy (n=82). Hypertrophic cardiomyopathy vs. Age- and sex-matched blood donors was evaluated on Peripheral plasma levels of 21 miRNAs and correlation with left ventricular hypertrophy and fibrosis. In patients with hypertrophic cardiomyopathy, 12 circulating miRNAs were significantly increased compared to controls, with only miR-29a significantly correlating with both hypertrophy and fibrosis.
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