Evaluation of NAT1 gene expression and methylation in the blood of people with breast cancer

Introduction: Breast cancer is one of the biggest health problems of women all over the world. Today, with the dramatic advancements made in this regard, we can see the increase in the life expectancy of women with this cancer. The current study aimed at investigating the NAT1 gene expression and methylation in the blood of people with breast cancer. This gene and its family, namely the group of NATs, especially NAT2 and NAT3, are one of the few genes that participate in the interaction of cancers such as breast by producing enzymes that carry factors such as oxygen and nitrogen, and their effect on carcinogens. Methods and Materials: In the current study, the rate of NAT1 gene expression is investigated by the use of the Real-time PCR technique. The methylation of NAT1 gene promoter is also evaluated by the use of MS PCR technique and blood samples of people with breast cancer and normal people among the Iranian population.