What question did this study set out to answer?

To clone, sequence, and evaluate the expression patterns of a ribosomal protein gene during dormancy release and growth arrest in pea axillary buds.

November 1, 1992PLANT PHYSIOLOGYOpen Access

Expression of a Ribosomal Protein Gene in Axillary Buds of Pea Seedlings

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To clone, sequence, and evaluate the expression patterns of a ribosomal protein gene during dormancy release and growth arrest in pea axillary buds.
Cloned and sequenced cDNA clone pGB8 (homologous to rat ribosomal protein L27) from decapitated pea seedlings (Pisum sativum L. cv Alaska).
Measured pGB8 transcript abundance across vegetative tissues and throughout repeated growth-dormancy cycles using RNA gel blot hybridization.
Localized tissue-specific mRNA using in situ hybridization and evaluated the inhibitory effect of indoleacetic acid (IAA at concentrations ≥10 µM) in cultured stem segments.
Decapitation triggered a 13-fold increase in pGB8 mRNA by 3 h, reaching a peak enhancement of approximately 35-fold at 12 h, with in situ hybridization detecting upregulation in all bud regions within 1 h.
Transcript levels fell back to dormancy levels upon growth arrest, although apical meristem expression remained elevated for 2 days after dropping in surrounding bud tissues.
Treatment with indoleacetic acid at concentrations ≥10 µM completely inhibited both bud outgrowth and pGB8 gene expression.

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Implication

Experimental study reveals rapid upregulation of a ribosomal protein gene during bud release in pea seedlings, indicating that protein synthesis machinery is swiftly activated upon breaking dormancy.

Cite This Study

Stafstrom et al. (1992) studied this question.

synapsesocial.com/papers/6a7cfaf06e07fdc915aeb6e7 https://doi.org/10.1104/pp.100.3.1494

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Implication

Experimental study reveals rapid upregulation of a ribosomal protein gene during bud release in pea seedlings, indicating that protein synthesis machinery is swiftly activated upon breaking dormancy.

Expression of a Ribosomal Protein Gene in Axillary Buds of Pea Seedlings

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Discussion

Implication

Cite This Study

Expression of a Ribosomal Protein Gene in Axillary Buds of Pea Seedlings

Key Points

Discussion

Implication

Cite This Study

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