Protoplasts have been prepared from normal and mutant lines of sycamore callus, maize root and tobacco leaf. Fusion was rare in sodium nitrate solutions. A mutant tissue culture was selected for by its resistance to 10 μg/ml bromodeoxyuridine. The mutant is sensitive to the Hypoxanthine-Aminopterin-Thymidine (HAT) selective medium but has normal thymidine transport and thymidine kinase activity.
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Bright et al. (1974) studied this question.
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