Answers are grounded in this paper and cite their sources.
Review demonstrates advances in three-dimensional imaging and optical clearing protocols in kidney tissue, highlighting improved quantification of complex renal morphology and disease.
Image analysis has played a critical role in our understanding of kidney morphology, function, and disease. This analysis has been historically limited to visualizing defined regions within the kidney in two dimensions. However, in recent years, significant advancements in microscopy have facilitated three-dimensional imaging and analysis of large tissue specimens and, in some cases, whole organs or organism. The use of these microscopy techniques combined with tissue-clearing strategies has resulted in detailed, multidimensional views of complex structures and processes within the kidney. This review discusses advanced light microscopy applications and optical clearing protocols that have been successfully modified for use in the kidney. Furthermore, this review will highlight how quantification of three-dimensional images has been applied in the kidney and thus contributed to novel spatiotemporal insights.
No takes yet. Share an insight, caveat, or question.
McLarnon et al. (2025) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: