Mucosa of the rat stomach was (fast) frozen rapidly by immersion in liquid nitrogen or Freon 22 before chemical fixation in glutaraldehyde and/or osmium tetroxide. In parietal cells, continuity between membranes of the canalicular system and tubulovesicles was seen. This confirms the hypothesis that membrane flow between the two elements occurs in the secretory cycle. Such continuity is difficult to demonstrate in material fixed directly and presumably, therefore, the rapid freezing was effective in preventing changes in membranes and membrane flow during the period of fixation.
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Thomas S. Leeson (1973) studied this question.
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