Antisera were generated against synthetic peptides that correspond to amino acid sequences deduced from a cDNA (designated beta 2) that encodes a second form of the beta subunit of guanine nucleotide-binding regulatory proteins (G proteins). The specificity of interactions of these antisera with purified G protein beta subunits indicates that the beta 2 cDNA encodes the beta 35 form of this polypeptide. This hypothesis is confirmed by the use of these antisera to detect expression of the beta 2 cDNA in COS-m6 cells.
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Gao et al. (1987) studied this question.
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