We describe here the simultaneous quantitation of diquat (DQ) and its two metabolites (DQ-monopyridone and -dipyridone) in serum and urine. Serum or urine was mixed vigorously with ten times volume of trichloroacetic acid and centrifuged at 12,000 rpm for 5 min. A 20 μl of the upper layer was injected into the HPLC. The major operating conditions of the HPLC were as follows: Column; ZorbaxR C8 (24cm × 4.6mm i.d.), Mobile phase; 5 % acetonitrile(v/v) containing ortho-phosphoric acid (0.2 M), diethylamine(0.1 M), and sodium octansulfonate(7.5 mM) and flow rate; 1.0 ml/min and UV detection by combined two UV detectors: 310 nm for DQ and 365 nm for DQ-monopyridone and DQ-dipyridone. Linear calibration curves for DQ, DQ-monopyridone and DQ-dipyridone were in the range of 0.1 – 10 μg/ml. In the serum and urine dosed intravenously DQ and its two metabolites were also detected.
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Ameno et al. (1995) studied this question.