Lynch (1915) first reported the successful cultivation of Trichomonas from the vagina and the mouth, and also from the intestine of man. A number of investigators later cultivated trichomonad flagellates from man and other animals; among them were Ohira and Noguchi (1917), Reuling (1918), Hogue (1921), Reichenow (1923) and Kofoid and Swezy (1923). Hegner and Becker (1922) and Reichenow (1923) suggested the advantage of the culture method in the diagnosis of trichomonas infections of man.
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Misao Tanabe (1925) studied this question.