A partially purified preparation of activated Hageman factor (factor XIIa) was prepared by anion exchange chromatography and gel filtration (Sephadex G-200) of noncontact human platelet poor plasma. The factor XIIa preparation corrected the clotting defect of congenitally factor XII-deficient plasma, but not that of plasma depleted of factor XI. It had a pI of approximately 5.3 and an approximate molecular weight of 118,000 by gel filtration and 86,000 by sucrose density gradient ultracentrifugation.
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Soltay et al. (1971) studied this question.