Saponins from sugar beet and the floc problem

Extraction of sugar beet (Beta vulgaris L.) molasses resulted in the isolation of the known 3-O-6-D-glucuronopyranosyl-3 beta-hydroxy-olean-12-en-28-oic acid (1) and a novel compound which was shown from mass and NMR spectroscopy to be 3-O-[beta-D-glucopyranosyl-(1-2) (beta-D-xylopyranosyl-(1-3))beta D-glucuronopyranosyl-3 beta-hydroxyolean-12-en-28-oic acid (2). These compounds are not present in the root of the fresh plant but are found in the leaves along with another new saponin: 3-0-[beta D-glucopyranosyl-(1-2)-(beta-D-xylopyranosyl-(1-3)) beta-D-glucuronopyranosyl]-28-0-beta-D-glucopyranosyl-3 beta-hydroxyolean-12-en-28-oic acid (3).