Summary. Crude preparations of collagenase, which have been used commonly for tissue dissociation, contain proteases that dissolve zonae pellucidae of hamster and mouse oocytes without reducing the ability of the oolemma to fuse with spermatozoa. This gentle proteolytic removal of zona is particularly useful for the study of sperm–oocyte fusion in mice, as trypsin, chymotrypsin and pronase damage the mouse oolemma.
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Zuccotti et al. (1991) studied this question.
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