Ministry of Agriculture, Fisheries and Food, Cattle Breeding Centre, Shinfield, Reading RG2 9BZ (Received 4th November 1974) One of the earliest visual signs of sperm damage is the loss of acrosomal integrity. Consequently, assay of the release into the seminal plasma of hyaluronidase, which is localized in the acrosome (Mancini, Alonsa, Bacquet, Avarez & Nemirosky, 1964; Gould & Bernstein, 1973) should provide an early and sensitive indication of damage sustained by the spermatozoa during processing of semen for artificial insemination (A.I.). Previous biochemical tests of sperm viability have included the assay of phenylalanine α-ketoglutarate transaminase released into boar seminal plasma (Gemert, Hendrike & Van der Horst, 1972). A correlation was found between high enzyme activity in seminal plasma and poor fertility. The release of glutamic oxaloacetic transaminase has been considered an indication of cell damage (Brown, Crabo, Graham & Pace, 1971) and assay of this non-acrosomal enzyme has been investigated
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Foulkes et al. (1975) studied this question.