Differences in rotation-mediated aggregation between wild-type and homozygous Brachyury (T) cells

Rotation-mediated aggregation was studied in dissociated cells from 9- and 10-day-old ('12-40 somite' stage) mouse embryos homozygous for the Brachyury (T) mutation. Average diameter of the aggregates formed by wild-type cells increased up to the 27-somite stage. In T/T cells, however, a completely different pattern was observed. The disappearance of free cells and the process of aggregate formation were also followed. The possibility that T/T cells have different adhesive properties from +/+ cells is discussed.