Key result
Metagenomic next-generation sequencing and transesophageal echocardiography successfully identified Cutibacterium acnes and Epstein-Barr virus as the causative agents of infective endocarditis.
Why the study?
Post-dialysis fever is a common but diagnostically challenging issue in hemodialysis patients, with diverse potential etiologies including cardiovascular complications.
Case Report (n=1)
TEE and NGS play a complementary role in diagnosing infective endocarditis in high-risk hemodialysis patients with culture-negative recurrent fever.
Answers are grounded in this paper and cite their sources.
Supports complementary NGS and TEE for culture-negative fever in hemodialysis patients with suspected endocarditis; hypothesis-generating for routine adoption.
BACKGROUND: Post-dialysis fever is a common but diagnostically challenging issue in hemodialysis patients, with potential causes including dialysis-related infections, pulmonary infections, and cardiovascular complications. CASE PRESENTATION: We report a 76-year-old male with end-stage renal disease (ESRD) on maintenance hemodialysis, coronary artery disease, and prior cardiac stent implantation, who presented with recurrent post-dialysis fever. Despite persistently negative conventional cultures, metagenomic next-generation sequencing (NGS) of pre-dialysis blood samples identified Pseudomonas aeruginosa (P. aeruginosa), Cutibacterium acnes (C. acnes; formerly Propionibacterium acnes), Staphylococcus epidermidis (S. epidermidis), and Corynebacterium accolens (C. accolens) and Epstein-Barr virus (EBV), while post-dialysis samples revealed only C. acnes and EBV. Given the temporal association with fever, these two pathogens were considered the primary causative agents. Subsequent transesophageal echocardiography (TEE) confirmed aortic valve vegetations, establishing the diagnosis of infective endocarditis (IE). Following targeted antimicrobial and antiviral adjustments based on NGS findings, the patient exhibited complete resolution of post-dialysis fever and was discharged. However, as the vegetation was not surgically removed, he was hospitalized multiple times over the following five months for recurrent infections and ultimately died of septic shock and multi-organ failure due to carbapenem-resistant Klebsiella pneumoniae. CONCLUSIONS: This case underscores the complementary role of TEE and NGS in diagnosing IE in high-risk patients, enabling the detection of uncommon pathogens and informing targeted therapy to improve clinical outcomes. CLINICAL TRIAL NUMBER: Not applicable.
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Wang et al. (2025) conducted a case report in Infective endocarditis (n=1). Metagenomic next-generation sequencing (NGS) and transesophageal echocardiography (TEE) was evaluated on Pathogen identification and diagnosis of infective endocarditis. Metagenomic next-generation sequencing and transesophageal echocardiography successfully identified Cutibacterium acnes and Epstein-Barr virus as the causative agents of infective endocarditis.
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