Characterization of Dn11 , a new Russian wheat aphid resistance gene in Iranian landrace PI 626575

This is the authors' abstract. We don't add key points for this paper.

Russian wheat aphid (RWA, Diuraphis noxia Kurdjumov) is a highly invasive and destructive wheat pest posing a threat to global wheat production, and host resistance is preferred for managing RWA because RWA can avoid insecticides by living inside rolled wheat leaves. However, RWA evolves quickly to overcome host resistance, necessitating continuous discovery of new resistance genes. PI 626575 is an Iranian landrace exhibiting resistance to RWA biotype 2 (RWA2), the dominant and most widely virulent RWA biotype in the United States. Evaluation of 131 F 2:3 lines from cross PI 626575 × Jagalene for responses to RWA2 infestation indicated that a single gene, designated Dn11 , confers RWA resistance in PI 626575. Genotyping of a subset of F 2 plants from the same cross using genotyping by sequencing led to identification of over 10,000 single nucleotide polymorphisms (SNPs). Allele distribution analysis of SNPs in the genotyped F 2 plants with contrasting phenotypes identified a set of SNPs associated with RWA resistance in the interval 401.7–578.8 Mb on chromosome arm 7DL. Linkage analysis using kompetitive allele‐specific PCR (KASP) markers converted from SNPs in the target region delimited Dn11 to an interval of 11.96 Mb between 519.22 and 531.18 Mb. Dn11 was 1.6 cM distal to Stars‐KASP1043 and 2.4 cM proximal to Stars‐KASP1046. A test of allelism indicated that Dn11 is a novel RWA resistance gene with loose linkage to Dn10 , previously mapped to chromosome arm 7DL. Estimated recombination frequency and genetic distance between Dn11 and Dn10 were 15.5 ± 0.6% and 16.04 ± 0.67 cM, respectively. Some KASP markers flanking Dn11 can be used to trace Dn11 in wheat breeding.