Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
March 1, 1995Journal of Biological ChemistryOpen Access

Potential Role of WAF1/Cip1/p21 as a Mediator of TGF-β Cytoinhibitory Effect

View Full Paper
Ask AI
Bookmark
Share

Authors

CLChuan‐Yuan LiDuke UniversityLSLaurent SuardetHarvard UniversityJLJohn B. LittleShepherd University

Discussion

Loading...

Member takes

Implication

In vitro study demonstrates that TGF-beta induces WAF1/Cip1/p21 to suppress cell cycle progression in human colon cancer cells, indicating its vital role in mediating cellular growth inhibition.

Key Points

  • To investigate whether the cyclin-dependent kinase inhibitor WAF1/Cip1/p21 mediates the cytoinhibitory and G1-phase arrest effects of transforming growth factor-beta in human colon cancer cells.
  • Treated TGF-beta-sensitive human colon cancer cell lines (LS1034 and LS513) and a TGF-beta-insensitive line (HT-29) with transforming growth factor-beta.
  • Evaluated WAF1/Cip1/p21 induction, dependence on p53 status, in vitro cyclin E-associated kinase activity, and in vivo retinoblastoma protein phosphorylation.
  • TGF-beta treatment induced WAF1/Cip1/p21 expression in growth arrest-sensitive LS1034 and LS513 cells—occurring independently of p53 in LS1034—whereas no induction occurred in insensitive HT-29 cells.
  • WAF1 physically associated with cyclin E, correlating with reduced cyclin E-associated kinase activity in vitro and inhibition of retinoblastoma protein phosphorylation in vivo.

Cite This Study

Li et al. (1995) studied this question.

synapsesocial.com/papers/6a7dcfd710b6f5f3737d9106https://doi.org/10.1074/jbc.270.10.4971
View Full Paper
Ask AI
Bookmark
Share