The addition of casein and lecithin to a phosphate-saline diluent in which ram and bull spermatozoa were cooled to 5°C increased their subsequent viability at 37°C and the effects were additive. Casein and lecithin also increased the metabolism of fresh ram semen. After storage at 5°C, oxygen uptake and oxidation of lactate by ram and bull spermatozoa were lower than before storage. The decrease in the amount of fructose oxidized to carbon dioxide was greater.
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O’Shea et al. (1966) studied this question.
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