Volhard-Harvey method (9), ammonia by the method of Folin and Bell (10) using a Klett-Summerson photom- eter, phosphate by the method of Lowry and Lopez (11), and titratable acidity by titrating to pH 7.4. The bi- carbonate concentration was calculated from the total CO, content and pH of each urine specimen by use of factors for CO, solubility and pK' in urine as functions of urinary total cation concentration, derived from Send- roy, Seelig, and Van Slyke (12).
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Barker et al. (1957) studied this question.