The structure of the naturally occurring phosphoglycerides. 2. Evidence derived from a study of the action of phospholipase C

Previous investigations on the structure of naturally occurring phosphoglycerides were ba mainly on the results of chemical hydrolysis X have been reviewed in the first paper of this sea (Long & Maguire, 1953a). It was pointed out t alkalis first split off the fatty-acid radicals so ae allow the formation of unstable cyclic phosph triesters from glycerylphosphorylcholine; th then lose choline, whereupon further splitting of diesters leads to the formation of isomeric glycE phosphates. The net effect is a partial migratior the phosphate group. However, by comparing proportions of ,8-, D-m-and L-a-glycerophosphE formed on alkaline hydrolysis of naturally occurr phosphoglycerides and of synthetic phosphoE cerides of known structure and optical configu CH2O .COR R'CO. OCH 0O + CH2O.PO. OCH2. CH2N