5′-Tailed Sequencing Primers Improve Sequencing Quality of PCR Products

Primer DetailsThe primers were designed to the desired length by adding a by adding a neutral 40-bp sequence (5′-AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA-3′) and a poly(C) tail to the 5′ end of the PCR primer (1,2). The neutral sequence is a randomly generated sequence not matching any sequence when a simple Basic Local Alignment Search Tool (BLAST) search is done in GenBank