Different yeast-like budding haploid strains of the yellow brain mushroom Tremella mesenterica (Retz.: Fr.) were obtained using the monobasidiosporous culture method from basidioma specimens collected in Israel. Nutritional requirements for biomass growth and extracellular polysaccharide production were investigated. A two-stage technology for acidic glucuronoxylomannan production was developed. On a first stage inoculum culture medium was balanced for biomass accumulation, and maximal polysaccharide production was obtained on a fermentation medium under conditions of nitrogen limitation. A crude product obtained by alcohol precipitation of culture broth contains 40% of acidic glucuronoxylomannan, 5% of neutral glucuronoxylomannan, 30% of cell biomass, and also includes low molecular weight substances, free amino acids, and mineral elements.
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Reshetnikov et al. (2001) studied this question.