Evaluation of antioxidant activities and inhibition effects of Tribulus terrestris L. extracts on some metabolic enzymes

This research aimed to determine the antioxidant capacity of Tribulus terrestris extracts made using various solvents and their effects on some metabolic enzymes . Five different antioxidant methods were used to assess the antioxidant capacities of the water, ethanol, acetone, chloroform, and hexane extracts of T. terrestris . The inhibitory effects of the extracts on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glycosidase enzymes and human carbonic anhydrase isoenzymes (hCA-I and hCA-II) were investigated. The study showed that acetone extract had the strongest inhibition effects in DPPH (IC 50 :25.86 ± 0.010 µg/mL) and ABTS (IC 50 :10.13 ± 0.023 µg/mL) radical scavenging activities. In CUPRAC/Fe 3+ -Fe 2+ /FRAP reduction tests, ethanol extract showed the highest reducing power. The ethanol extract exhibited the highest level of inhibition for the AChE/BChE and α -glycosidase enzymes (IC 50 :12.13 ± 0.37 µg/mL, IC 50 :22.33 ± 0.27 µg/mL and IC 50 :25.98 ± 0.75 µg/mL), respectively. Chloroform extract showed the strongest inhibition effect on hCA-I/hCA-II isoenzymes (IC 50 :21.82 ± 0.55 µg/mL, IC 50 :20.97 ± 0.63 µg/mL), respectively.