A method of estimating volatile fatty acid (VFA) production in the rumen in non-steady-state conditions was proposed. Laboratory models indicated that good estimates of total production over a complete feeding cycle could be obtained even when a wide range of production rates and volume changes occurred during the experiment. But estimates of instantaneous production rates might have contained large errors unless some attempt was made to match the infusion rate of labelled VFA to the rate at which VFA was produced in the rumen.
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Morant et al. (1978) studied this question.
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