Gut microbiome-mediated nutrients alter opportunistic bacterial growth in peritonitis

Peritonitis induced via cecal slurry or LPS induced similar changes in prevalent metabolites in the murine peritoneal microenvironment, increasing glutamine and pyruvate and decreasing glucose and butyrate. Gut microbiome depletion using germ-free mice or antibiotic pretreatment blunted many of these peritoneal metabolite changes. Peritoneal fluid from microbiome-depleted LPS-treated mice exhibited reduced bacterial growth of two Enterobacteriaceae commensals in an ex vivo peritonitis model compared with fluid from conventional LPS-treated animals.