Summary Sandwich enzyme immunoassay of Mucor miehei proteinase (from the commercial preparation Fromase) has been developed using horseradish peroxidase as a marker. The enzyme may be determined to a detection limit of 50 ng/ml. Slight cross reactions by chymosin, bovine, porcine and chicken pepsin did not affect the assay. Recovery of Fromase added to cheese extracts varied between 92 and 106%. The method was used for the determination of M. miehei proteinase in different cheese extracts.
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Rauch et al. (1989) studied this question.
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