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Bioassay-directed fractionation of a Toddalia asiatica (Rutaceae) extract concentrated anti-HIV activity into fractions rich in quaternary alkaloids. Nitidine (1), a benzo[c]phenanthridine alkaloid, and magnoflorine (2), an aporphinoid alkaloid, have been isolated and identified from these fractions. In an in vitro XTT-based anti-HIV assay, 1-10 μ/ml nitidine (1) inhibited human lymphoblastoid cell killing by HIV-1.
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Rashid et al. (1995) studied this question.
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