The feasibility of using nonradioactive heterologous serum albumin measured by rocket immunoelectrophoresis as tracer for plasma volume measurements was evaluated by comparing simultaneous distribution volumes and plasma half-lives (t1/2) of human serum albumin (HSA) and Evans blue dye (T1824) in a group of conscious rats. The animals were studied under normal water intake and after a 48-h period of water deprivation. The kinetic behavior in plasma was qualitatively similar for both tracers, but T1824 distributed beyond the HSA space and had half-lives in plasma lower than those of HSA. Water deprivation induced a 14% decrease in the HSA space, from 2.7 +/- 0.1 to 2.4 +/- 0.2 ml/100 g body weight. We conclude that serum albumin spaces measured immunoelectrophoretically are a better reflection of true plasma volume than T1824 spaces and can be used advantageously in determinations of plasma volume.
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Jaime S. Carvalho (1989) studied this question.