Key result
In vivo native proplatelet elongation is regulated by myosin-IIA and is less dependent on microtubules than in vitro cultured proplatelets, with blood flow forces likely contributing to extension.
Why the study?
Contrary to cultured proplatelets, the mechanisms regulating native proplatelet formation by bone marrow megakaryocytes are poorly understood.
Population
Living mice, including WT, β1-tubulin-deficient, and myosin-deficient mice
Comparison
Native vs cultured proplatelet formation across genetic models and microtubule depolymerization
Design
Preclinical animal and in vitro mechanistic study
Authors
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In vitro assays may poorly guide thrombocytopenia therapies; challenges microtubule-centric models and leaves open human in vivo mechanisms.
The mechanisms of proplatelet formation differ significantly between in vivo and in vitro environments, with blood flow forces playing a major role in vivo.
Bornert et al. (2020) studied Thrombopoiesis. In vivo bone marrow environment vs. In vitro culture was evaluated on Proplatelet elongation dynamics and cytoskeletal dependence. In vivo native proplatelet elongation is regulated by myosin-IIA and is less dependent on microtubules than in vitro cultured proplatelets, with blood flow forces likely contributing to extension.
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