Pitfalls in Measuring Cytokines

Letters15 July 1994Pitfalls in Measuring CytokinesLarry C. Case, MD, PhD, Robert Balk, MD, and Roger Bone, MDLarry C. Case, MD, PhD, Robert Balk, MD, and Roger Bone, MDAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-121-2-199407150-00017 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:Numerous assays are available to investigators for measuring cytokines, and a study by Parson and colleagues [1] shows that different assays yield different results. Which is correct? Why are there differences? The data from Dr. Mann and colleagues show that the presence of circulating soluble receptors to TNF may interfere with TNF immunoassays, depending on the assay kit. In the data presented, 500 ng/mL of the circulating soluble receptors resulted in the loss of detection of TNF by using the assay kit we used. However, the concentration of these receptors in their study was approximately 50 times higher ...REFERENCE1. Parsons PE, Moore FA, Moore EE, Ikle DN, Henson PM, Worthen GS. Studies on the role of tumor necrosis factor in adult respiratory distress syndrome. Am Rev Respir Dis. 1992; 146:694-700. Google Scholar Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byPlasma Cytokine Parameters and Mortality in Patients With Chronic Heart FailureTumor Necrosis Factor-α and Tumor Necrosis Factor Receptors in the Failing Human HeartTumor necrosis factor-α and the failing human heart-TNFα and heart failure 15 July 1994Volume 121, Issue 2Page: 149-150KeywordsAttentionBioassays and physiological analysisCellsCytokinesImmunoassaysMortalitySepsisT cell receptorsT cellsTumor necrosis factor Issue Published: 15 July 1994 Copyright & PermissionsCopyright © 1994 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...