2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses

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  Fig. 1.. Broad-spectrum antiviral activities of s2U against Flaviviruses and CHIKV. (A) Chemical structure of 2-thiouridine (s2U). (B and C) Dose-response inhibition of DENV2 replication by s2U in VeroE6 (B) and Huh7 (C) cells. Cell lysates were collected for viral RNA determination, and viral RNA levels were determined relative to ACTB transcripts. (D) Dose-response inhibition of DENV2 propagation by s2U. Supernatants of DENV2-infected BHK-21 cells were collected at 72 hours post-infection (hpi), and dilutions were used to inoculate BHK-21 cells. Four days after inoculation, viral titers were determined by the plaque assay. (E–I) Dose-response inhibition of ZIKV (E), YFV (F), JEV (G), WNV (H), and CHIKV (I) by s2U. Cell lysates were collected for viral RNA determination, and viral RNA levels were determined relative to ACTB transcripts. (J) Dose-response inhibition of viral protein expression in the DENV2- and CHIKV- infected cells. Cells were stained with viral-specific antibodies (green, DENV2: Envelope protein, CHIKV: E1 protein) and counterstained with Hoechst 33342 nuclear dye (blue). (Scale bars indicate 200 μm.) Data are presented as mean values of biological triplicates from one…

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  Fig. 2.. Broad-spectrum antiviral activities of s2U against human coronaviruses, including SARS-CoV-2. (A–K) Dose-response inhibition of HCoV-229E (A), HCoV-OC43 (B), SARS-CoV (C), MERS-CoV (D), and several SARS-CoV-2 variants (E–K) by s2U. Cell lysates were collected for viral RNA determination, and viral RNA levels were determined relative to ACTB transcripts. (L) Dose-response inhibition of viral protein expression in the SARS-CoV-2-infected cells. Cells were stained with viral-specific antibodies (green, Nucleocapsid) and counterstained with Hoechst 33342 nuclear dye (blue). (Scale bars indicate 200 μm.) Data are presented as mean values of biological triplicates from one of the experiments, and error bars indicate the SD. Statistically significant differences were determined using a one-way ANOVA followed by Dunnett’s multiple comparisons test to compare with non-treated cells; *P < 0.01, **P < 0.005, ***P < 0.0005, and ****P < 0.0001.

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  Fig. 3.. Molecular target and mechanism of action of s2U. (A) Ribonucleoside competition of DENV2 inhibition by s2U. DENV2 [multiplicity of infection (MOI) = 0.01]-infected BHK-21 cells were treated with 10 μM of s2U and serial dilutions of exogenous nucleosides. A resazurin reduction assay was performed at 4 days post-infection (dpi). (B) Schematic presentation of the experimental design for drug-escape mutant selection and sequence result. BHK-21 cells were infected with DENV2 in the presence of s2U. The passage of infected cells or culture supernatant was performed every 2 to 3 d. Base substitution was detected using Sanger sequencing. (C) Effect of s2U resistance mutation on anti-DENV2 activity of s2U. BHK-21 cells were infected with rgDENV2-WT or rgDENV2-NS5-G605V (MOI = 0.1) containing a serially diluted compound. A resazurin reduction assay was performed at 4 dpi. (D) Analysis of viral RdRp stalling by s2U 5′-triphosphate (s2UTP). Denaturing polyacrylamide gel electrophoresis fraction of RNA transcripts produced through primer extension by ZIKV RdRp in the presence of the indicated nucleotides. The RNA primer/template sequence used in this assay is indicated at the top (small black…

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  Fig. 4.. In vivo efficacy of s2U in the DENV2 and SARS-CoV-2 mouse model. (A) Schematic representation of the survival and viremia studies using AG129 mice and strain DENV2 AG-P10. (B) Effect of s2U on survival of DENV2 AG-P10-infected (1 × 102 plaque-forming units (PFU)] mice (7-wk-old, female) orally treated twice daily with s2U (50 or 150 mg/kg) compared with vehicle-treated mice. Treatment started immediately after infection. Data are from two independent studies with 9 (in total, vehicle and 150 mg/kg) or 8 (in total, 50 mg/kg) mice per group. (C) Effect of s2U on survival of DENV2 AG-P10-infected (1 × 102 PFU) mice (7-wk-old, female) orally treated twice daily with s2U (150 mg/kg) compared with vehicle-treated mice. Treatment started 8 or 24 h after infection (n = 5 per group). (D) Effect of s2U on viremia at 3 dpi in mice treated twice daily with s2U (50 or 150 mg/kg) compared with vehicle-treated mice (n = 5 per group, 7-wk-old, male). Viral RNA copies/mL of serum samples were quantified using qRT-PCR. (E) Schematic representation of the survival and viremia studies using BALB/c mice and SARS-CoV-2 MA-P10. (F–I) Effect of s2U on survival and body weight change in SARS-CoV-2…