Flavonoids in cultivated berries of sea buckthorn (Hippophaë rhamnoides L.)

Flavonoids of sea buckthorn ( Hippophaë rhamnoides L.) possess a wide spectrum of physiological activities on cardiovascular system [1,2]. The content and profile of flavonoids of certain cultivars grown in Lithuania are unknown. This study investigated flavonoids of 11 cultivars of sea buckthorn ( H. rhamnoides L.) “Avgustinka”, “Botaničeskaja”, “Botaničeskaja Liubitelskoje”, “Hibrid perčika”, “Julia”, “Nivelena-1”, “Nivelena-2”, “Otradnaja”, “Padarok sadu”, “Trofimovskaja”, “Vorobjevskaja“ by reverse phase high performance liquid chromatography combined with diode array detection. Quercetin-3-O-rutinoside, quercetin-3-O-glucoside, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-glucoside, quercetin and isorhamnetin were identified and quantified in all tested samples. The predominant flavonoids were quercetin-3-O-rutinoside (99 – 280 µg/g fw), isorhamnetin-3-O-rutinoside (102 – 229 µg/g fw) and isorhamnetin-3-O-glucoside (83 – 195 µg/g fw). The total content of flavonoids fell in the range of 385 – 616 µg per gram fresh berries with considerable variation. Significantly greatest amounts of flavonoids were detected in “Avgustinka” cultivar, whereas lowest amounts – in “Nivelena-1” cultivar. In conclusion, the content and profile of flavonoids in sea buckthorn berries are dependent on the cultivars. Acknowledgements: Postdoctoral fellowship is being funded by the European Union Structural Funds project (VPl-3.1-ŠMM-01-V-02 – 004 No. 004/86) “Postdoctoral Fellowship Implementation in Lithuania”.