Key result
Transgenic beta-MHC expression in mice worsens LV hypertrophy, decompensation, and dilation under severe cardiovascular stress.
Why the study?
A shift from alpha- to beta-MHC occurs in failing hearts, but its impact on disease progression and cardiac function under chronic stress is not well understood.
Does a pre-existing shift from alpha- to beta-MHC affect cardiac function under chronic mechanical or pharmacologic cardiovascular stress in mice?
Does a pre-existing shift from alpha- to beta-MHC affect cardiac function under chronic mechanical or pharmacologic cardiovascular stress in mice?
The shift from alpha- to beta-myosin heavy chain isoform observed in cardiac disease appears to be a maladaptive response that worsens cardiac function under severe cardiovascular stress.
OBJECTIVES: In failing mouse and human hearts, a shift in myosin heavy chain (MHC) isoform content from alpha to beta can occur. However, the impact of this phenomenon on disease progression is not well understood. Therefore, using transgenic (TG) mice, we tested how a pre-existing shift from alpha- to beta-MHC affects cardiac function under chronic mechanical or pharmacologic cardiovascular stress. BACKGROUND: Expression of beta-MHC is considered to be energetically favorable, but this might be offset by depressed cardiac function. METHODS: Transgenic mice with near-complete replacement of the normally predominant alpha- with beta-MHC were subjected to cardiac stress. RESULTS: At baseline, TG mice show moderately reduced cardiac contractile function but are otherwise healthy with normal ventricular morphology. After four weeks of swimming, both TG and non-TG animals showed a 20% increase in left ventricular (LV)/body weight ratios. The TG hearts displayed mildly greater end-diastolic and end-systolic LV diameters than nontransgenic hearts after training, but no signs of LV failure were observed. However, chronic stimulation with isoproterenol resulted in augmented LV hypertrophy with signs of LV decompensation in TG mice. Furthermore, in a post-infarction failure model, TG hearts displayed accelerated LV dilation and a faster decline of shortening fraction. CONCLUSIONS: Expression of beta-MHC appears to be disadvantageous to the mice under severe cardiovascular stress, implying that the alpha-->beta-MHC isoform shift observed in cardiac disease may be a maladaptive response.
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Krenz et al. (2004) studied Cardiovascular stress. Transgenic beta-myosin heavy chain (beta-MHC) expression vs. Non-transgenic mice was evaluated on Cardiac function and ventricular morphology under stress. Transgenic expression of beta-MHC in mice resulted in augmented LV hypertrophy, decompensation, and accelerated dilation under severe cardiovascular stress compared to non-transgenic mice.
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