Human Amniotic Fluid Mesenchymal Stem Cell‐Derived Exosomes Inhibit Apoptosis in Ovarian Granulosa Cell via miR‐369‐3p/YAF2/PDCD5/p53 Pathway

Human amniotic fluid stem cell‐derived exosome (HuAFSC‐exosome) transplantation is considered a promising treatment for premature ovarian failure (POF). However, its mechanism remains unclear. In this study, exosomes were isolated and enriched from HuAFSC subsets of CD44+/CD105+, and the exosomes were transplanted into a POF model in vitro and in vivo . Our results confirmed that the exosomes produced by CD44+/CD105+ HuAFSCs could achieve therapeutic effects in a mouse POF model. Our research also showed that CD44+/CD105+ HuAFSC‐exosomes carrying miR‐369‐3p could specifically downregulate the expression of YAF2, inhibit the stability of PDCD5/p53, and reduce the apoptosis of ovarian granulosa cells (OGCs), thereby exerting therapeutic effects on POF. Knowledge of these mechanisms demonstrates that miRNAs carried by CD44+/CD105+ HuAFSC‐exosomes are critical to the therapy of POF. This will be useful for the clinical application of stem cells.