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The reversed-phase chromatography of triglycerides is very sensitive to both changes in the properties of the injection solvent relative to those of the mobile phase and to the volume injected. These well-established phenomena are rationalised in terms of solvent parameters and suggestions are made as to how optimum conditions can be derived. The practical consequences of this are illustrated with chromatograms of olive oil samples.
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Tsimidou et al. (1985) studied this question.
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