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A sequence coding for the transit peptide part of the precursor of the ribulose bisphosphate carboxylase small subunit can be used to construct chimaeric genes coding for easily detectable marker proteins. Chloroplasts of transgenic plants containing such chimaeric genes were found to contain the marker proteins. In addition, measurements of uptake by isolated chloroplasts of various precursor proteins, synthesized in vitro, demonstrated that most if not all of the transit peptide sequence was necessary and sufficient to obtain transport into chloroplasts. However, quantitative measurements of uptake indicated that high efficiency of transport required domains from both the transit peptide and the mature small subunit protein.
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Schreier et al. (1986) studied this question.
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