Combination of Morroniside and Diosgenin Prevents High Glucose-Induced Cardiomyocytes Apoptosis

Figures

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  Figure 1. Effects of Mor (25, 50, 100 μg/mL), Dio (25, 50, 100 μg/mL), and M + D (25, 50, 100 μg/mL) on rat cardiomyocyte viability under HG-induced cell injury after pretreated 72 h. Values are expressed as the mean ± SD. ## p < 0.01 vs. CC; ** p < 0.01, * p < 0.05 vs. HG.

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  Figure 2. Effect of Mor (25, 50, 100 μg/mL), Dio (25, 50, 100 μg/mL), and M + D (25, 50, 100 μg/mL) on HG-induced cardiomyocyte apoptosis. (M + D)H significantly prevents from cardiomyocytes apoptosis as determined with TUNEL staining. Data are expressed as mean ± SD. ## p < 0.01 vs. CC; ** p < 0.01, vs. HG; aa p < 0.01, vs. MorH; bb p < 0.01, vs. DioH.

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  Figure 3. (M + D)H protects cardiomyocytes from mitochondrial production ROS induced with HG as determined with DCFH staining. Data are expressed as mean ± SD. ## p < 0.01 vs. CC; ** p < 0.01, vs. HG; aa p < 0.01, vs. MorH; bb p < 0.01, vs. DioH.

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  Figure 4. Regulation of proteins Bcl-2 and Bax associated with apoptosis by MorH, DioH, and (M + D)H. Data are expressed as mean ± SD. ## p < 0.01 vs. CC; ** p < 0.01, vs. HG; aa p < 0.01, vs. MorH; bb p < 0.01, vs. DioH.

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  Figure 5. (A) Effect of (M + D)H on caspase-3 activity by using a colorimetric activity assay kit. Data are expressed as mean ± SD. ## p < 0.01 vs. CC; ** p < 0.01 vs. HG; aa p < 0.01, vs. MorH; b p < 0.05 vs. DioH; and (B) the effect of (M + D)H on the expression levels of caspase-3 protein of cardiomyocytes after HG injury. Data are expressed as mean ± SD. ## p < 0.01 vs. CC; ** p < 0.01 vs. HG; aa p < 0.01, vs. MorH; b p < 0.05 vs. DioH.