Seven components have been demonstrated chromatographically in crystalline preparations of egg-white lysozyme. This was effected with a chromatographic procedure employing BioRex 70 columns eluted with 0.2 M potassium phosphate buffer, pH 7.09. Six components possess enzymic activity and 3 have molecular weights very close to that of the major chromatographic component. The potential danger of assuming homogeneity in crystalline lysozyme preparations is indicated, particularly in regard to the study of radiation products.
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Stevens et al. (1967) studied this question.