Dual-wavelength filtering for the detection of blood-spotted eggs

This study developed an image-based dual-wavelength filter method for detecting blood-spotted white-shelled eggs based on transmission spectral analysis. The spectral results identified a pronounced absorption feature near 580 nm in blood-spotted eggs. Accordingly, 580 nm was selected as the blood-sensitive wavelength and 630 nm as the reference wavelength, and the dual-wavelength image intensity ratio was used for detection. Binary logistic regression models were constructed for both the image-based dual-wavelength filter method and the transmission spectroscopy method. The models were evaluated using artificially prepared blood-spotted white-shelled egg samples containing 0.025 and 0.05 mL of blood, together with commercially sourced blood-spotted white-shelled eggs. The retrained image-based dual-wavelength method achieved 84.9% overall accuracy and 83.8% sensitivity for the commercially sourced samples. These results indicate that the proposed method has potential for nondestructive screening of blood-spotted white-shelled eggs under static laboratory conditions.