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June 28, 2012Proceedings of the National Academy of SciencesOpen Access

An I(Kr) chemical activator that reduces voltage sensitivity of inactivation caused dose-dependent shortening of action potential durations and normalized action potentials in LQT1 cells.

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Why the study?

Does a specific I(Kr) chemical activator normalize action potential duration in human cardiomyocytes from patients with LQT1?

Population

Cultured differentiated human cardiomyocytes from healthy controls and patients with Long QT syndrome type 1

Comparison

Specific chemical activator for I causing a… vs I chemical activator of primary effects in…

Design

Preclinical

Key result

An I(Kr) chemical activator that reduces voltage sensitivity of inactivation caused dose-dependent shortening of action potential durations and normalized action potentials in LQT1 cells.

Authors

HZHongkang ZhangBZBeiyan ZouHYHaibo Yu

Discussion

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Member takes

Overview

Should not yet change LQT1 practice; leaves open I(Kr) activation as a therapeutic target pending human validation.

Structured PICO

Does a specific I(Kr) chemical activator normalize action potential duration in human cardiomyocytes from patients with LQT1?

P
Population
Cultured differentiated human cardiomyocytes from healthy controls and patients with Long QT syndrome type 1 (LQT1)
I
Intervention
Specific chemical activator for I(Kr) causing a right shift of V(1/2) for inactivation
C
Comparator
I(Kr) chemical activator of primary effects in slowing channel deactivation
O
Outcome
Action potential durationsurrogate

Pharmacological elevation of I(Kr) by reducing voltage sensitivity of inactivation can normalize action potential duration in LQT1 cardiomyocytes, providing a theoretical and experimental basis for novel LQTS therapies.

Cite This Study

Zhang et al. (2012) studied Long QT syndrome type 1 (LQT1). I(Kr) chemical activator causing a right shift of V(1/2) for inactivation vs. I(Kr) chemical activator slowing channel deactivation was evaluated on Action potential duration. An I(Kr) chemical activator that reduces voltage sensitivity of inactivation caused dose-dependent shortening of action potential durations and normalized action potentials in LQT1 cells.

synapsesocial.com/papers/6a81813bd433ecbb06e1d183https://doi.org/10.1073/pnas.1205266109
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