Expression of Fluorescent Cyclotides using Protein Trans‐Splicing for Easy Monitoring of Cyclotide–Protein Interactions

Going in circles: Expression of cyclotides containing non-natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing p-azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper-free click chemistry to monitor the cyclotide–protein interactions.