What question did this study set out to answer?

To clone and characterize the cDNA encoding a tobacco DNA-binding protein that binds the light-responsive box II cis-element of the rbcS promoter.

July 1, 1992The Plant CellOpen Access

A tobacco DNA binding protein that interacts with a light-responsive box II element.

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Authors

OPOlga PerišićMRC Laboratory of Molecular Biology ELEric LamRutgers, The State University of New Jersey

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Implication

Molecular study reveals a tobacco DNA-binding factor interacting with light-responsive rbcS promoter elements, highlighting conserved structural motifs in plant transcriptional regulation.

Key Points

To clone and characterize the cDNA encoding a tobacco DNA-binding protein that binds the light-responsive box II cis-element of the rbcS promoter.
Cloned and characterized a tobacco cDNA encoding Box II Factor (B2F).
Assessed DNA-binding specificity and complex mobility using gel retardation assays alongside native nuclear factor GT-1.
Measured steady-state B2F mRNA expression in light-grown versus dark-adapted tissues and compared amino acid sequence homology with rice factor GT-2.
B2F displayed identical binding specificity and electrophoretic mobility in gel retardation assays to nuclear factor GT-1.
Steady-state levels of B2F mRNA did not change in response to light, matching the constitutive activity of native GT-1 in both light and dark conditions.
Sequence comparison revealed high homology between B2F and rice GT-2 across three putative alpha-helices predicted to mediate DNA binding.

Cite This Study

Perišić et al. (1992) studied this question.

synapsesocial.com/papers/6a81f29ee5ad2a206e4d552e https://doi.org/10.1105/tpc.4.7.831

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Implication

Molecular study reveals a tobacco DNA-binding factor interacting with light-responsive rbcS promoter elements, highlighting conserved structural motifs in plant transcriptional regulation.

A tobacco DNA binding protein that interacts with a light-responsive box II element.

Discussion

Member takes

Implication

Key Points

Cite This Study

A tobacco DNA binding protein that interacts with a light-responsive box II element.

Discussion

Member takes

Implication

Key Points

Cite This Study

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