A simple procedure is formulated to correct for the Johnston-Ogston effect which operates in the velocity sedimentation of mixtures comprising two components. The procedure is applied to the analysis of results obtained with proteoglycan mixtures from bovine nasal cartilage. It is shown that the method permits the evaluation of the true concentration ratio of the components from velocity sedimentation data ob-tained at two different total concentrations. In the preparation of proteoglycans, it often occurs that materials obtained are heteroge-neous, containing several discrete components which are not easily separable from one an-other. Sedimentation analysis of such mix-tures, though potentially capable of providing valuable information, is complicated by the concentration anomaly caused by the operation of the Johnston-Ogston effect ( /). The anal-
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Janado et al. (1972) studied this question.